Terms I didn’t remember/know:

  • Alpha amylases: Hydrolyse alpha bonds of polysaccharides (e.g. starch, glycogen) into monosaccharides (e.g. glucose, maltose)
  • Nucleotide excision repair: Cellular mechanism that repairs DNA damage by removing bulky, helix distorting lesions
  • Base Excision Repair (BER): Repairs DNA damage by removing small, non helix distorting lesions
  • Homologous recombination: Nucelotide sequences are exchanged between two similar or identical molecules of DNA. Usuallu used to repair ds breaks.
  • Resolvases: Nuclease involved in DNA recombination.
  • Holliday junction: Cross shaped structure formed when two ds DNA strands are separated into four strands to exchange segments of genetic info.
  • Mitomycin C (MMC): Antibiotic that crosslinks complementary DNA strands with selectivity to the N2 of guanine.
  • Cross linking: A covalent linkage formed between two DNA nucleotides. Can occur between same strands (intrastrand) or opposite strands (interstrand) of ds DNA.
  • Cell viability: No. of healthy cells in a sample. Determines amount of living or dead cells based on a total sample
  • UvrD: A helicase that unwinds DNA duplexes in 3’ to 5’ polarity (according to bound strand). Involved in post incision events of nucleotide excision repair.
jul 19 2018 ∞
jan 29 2019 +