- DNA synthesis
- Model ydgJ/nrdD binding
- How ydgJ influences nrdD
- nrdD synthesises deoxynucleotides = direct SOS response to DNA repair (nor cell death)
- Replication/transcription proteins
- Many in mass spec
- May be enough to overcome SOS response
- Could be to do with ydgJ unregulated synthesis
- Outer membrane proteins
- ydgJ/ArnA interactions
- Mention TMD possibility
- Alter LPS so nal cannot enter
- Link to polymyxin resistance mechnaism
- ETC
- Model ydgJ with ETC proteins
- ydgJ = oxidoreductase
- So may have similar activity to ETC proteins
- Mention TMD / association with TMD
- May interact with proteins of similar function
- Reduce ETC protein activity = lower free radicals
- But may only interact due to similar function (not resistance)
- ydgJ/DNAK
- DNAK = highest sequence identity
- Implies most likely to be mass spec match
- Could protect proteins from nal attack
- E.g. membrane proteins (example), or topoisomerases
- But chaperone / heat shock protein
- May unregulated in response to stress from nal
- Present if ydgJ unregulated to aid folding
mar 12 2019 ∞
apr 20 2019 +